Methods: To make the severe E, Coli sepsis model of mouse, it was treated by the method of bacteria, endotoxin, inflammatory mediator treated simultaneously, to observe whether the survival rate of severe E. Coli sepsis can be improved with this method. 方法:制作重症E.Coli脓毒病小鼠模型,用细菌、内毒素、炎性介质并治的方法治疗,观察此方法是否能提高重症E.Coli脓毒病小鼠存活率。